Anti-DOCK180 antibody (ab76927)
Key features and details
- Rabbit polyclonal to DOCK180
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK180 antibody
See all DOCK180 primary antibodies -
Description
Rabbit polyclonal to DOCK180 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human DOCK180.
Database link: NP_001371.1 -
Positive control
- HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab76927 was affinity purified using an epitope specific to DOCK180 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 215 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 215 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. -
Tissue specificity
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon. -
Sequence similarities
Belongs to the DOCK family.
Contains 1 DHR-1 (CZH-1) domain.
Contains 1 DHR-2 (CZH-2) domain.
Contains 1 SH3 domain. -
Domain
The DHR-2 domain is necessary and sufficient for the GEF activity. -
Cellular localization
Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate. - Information by UniProt
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Database links
- Entrez Gene: 1793 Human
- Entrez Gene: 330662 Mouse
- Entrez Gene: 309081 Rat
- Omim: 601403 Human
- SwissProt: Q14185 Human
- SwissProt: Q8BUR4 Mouse
- Unigene: 159195 Human
- Unigene: 436572 Mouse
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Alternative names
- 180 kDa protein downstream of CRK antibody
- Ced5 antibody
- Dedicator of Cytokinesis 1 antibody
see all
Images
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All lanes : Anti-DOCK180 antibody (ab76927) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 215 kDa
Observed band size: 215 kDa
Exposure time: 3 minutes -
Detection of DOCK180 by Western Blot of Immunprecipitate.
ab76927, at 1µg/ml, staining DOCK180 in HeLa whole cell lysate immunoprecipitated using ab76927 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76927 has been referenced in 1 publication.
- Teng S et al. The Protection of Crocin Against Ulcerative Colitis and Colorectal Cancer via Suppression of NF-?B-Mediated Inflammation. Front Pharmacol 12:639458 (2021). PubMed: 33841156