Overview

  • Product name
  • Description
    Rabbit polyclonal to DOCK1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1852 and 1865 of Human DOCK1 (NP_001371).

  • Positive control
    • WB: 293T whole cell lysate; HeLaS3 cell lysate ICC/IF: A431 cells IHC-P: Lung CA

Properties

Applications

Our Abpromise guarantee covers the use of ab97325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 215 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
  • Tissue specificity
    Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
  • Sequence similarities
    Belongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.
    Contains 1 SH3 domain.
  • Domain
    The DHR-2 domain is necessary and sufficient for the GEF activity.
  • Cellular localization
    Cytoplasm. Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-triphosphate.
  • Information by UniProt
  • Database links
  • Alternative names
    • 180 kDa protein downstream of CRK antibody
    • Ced5 antibody
    • Dedicator of Cytokinesis 1 antibody
    • Dedicator of cytokinesis protein 1 antibody
    • DOCK 1 antibody
    • DOCK 180 antibody
    • DOCK1 antibody
    • DOCK1_HUMAN antibody
    • DOCK180 antibody
    see all

Images

  • Anti-DOCK1 antibody (ab97325) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 215 kDa
  • Immunofluorescence analysis of DOCK1 in methanol fixed A431, using ab97325 at a 1/200 dilution.
  • Immunohistochemical analysis of DOCK1 in paraffin embedded Lung CA, using ab97325 at a 1/100 dilution.

References

This product has been referenced in:
  • Toret CP  et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). Read more (PubMed: 25452388) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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