Overview

  • Product name
  • Description
    Rabbit polyclonal to DOCK10
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 100 and 150 of human DOCK10 (NP_055504.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    DOCK10 is a potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP. Two named isoforms exist, produced by alternative splicing.
  • Database links
  • Alternative names
    • 9330153B10Rik antibody
    • A630054M16Rik antibody
    • dedicator of cytokinesis 10 antibody
    • Dedicator of cytokinesis protein 10 antibody
    • DKFZp781A1532 antibody
    • DRIP2 antibody
    • Jr4 antibody
    • Jr5 antibody
    • KIAA0694 antibody
    • MGC183664 antibody
    • mKIAA0694 antibody
    • Nbla10300 antibody
    • R75174 antibody
    • ZIZ3 antibody
    • Zizimin 3 antibody
    see all

Anti-DOCK10 antibody images

  • All lanes : Anti-DOCK10 antibody (ab75258) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 250 kDa
    Observed band size : 250 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Detection of DOCK10 by Western Blot of Immunprecipitate.
    ab75258 at 1µg/ml staining DOCK10 in HeLa whole cell lysate immunoprecipitated using ab75258 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: chemiluminescence with exposure time of 1 minute.

References for Anti-DOCK10 antibody (ab75258)

ab75258 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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