Overview

  • Product nameAnti-DOCK2 antibody
    See all DOCK2 primary antibodies
  • Description
    Rabbit polyclonal to DOCK2
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human DOCK2.

    (Peptide available as ab74658.)

  • Positive control
    • This antibody gave a positive signal in Jurkat Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab74659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 212 kDa (predicted molecular weight: 212 kDa).

Target

  • RelevanceDOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
  • Cellular localizationIntracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
  • Database links
  • Alternative names
    • Dedicator of cyto kinesis 2 antibody
    • Dedicator of cytokinesis 2 antibody
    • Dedicator of cytokinesis protein 2 antibody
    • DOCK 2 antibody
    • FLJ46592 antibody
    • KIAA0209 antibody
    see all

Anti-DOCK2 antibody images

  • Anti-DOCK2 antibody (ab74659) at 2 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 212 kDa
    Observed band size : 212 kDa
    Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC image of ab74659 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-DOCK2 antibody (ab74659)

ab74659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"