Anti-DOCK2 antibody [EPR6647] (ab124838)

Overview

  • Product nameAnti-DOCK2 antibody [EPR6647]
    See all DOCK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6647] to DOCK2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of Human DOCK2 protein (Q92608).

  • Positive control
    • Jurkat and Raji cell lysates and Human lung tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 212 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is not recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • RelevanceDOCK2 (dedicator of cytokinesis 2) is a hematopoietic cell-specific protein that participates in the cytoskeletal rearrangements required for lymphocyte migration in response to chemokines. DOCK2 activates the small GTPases RAC1 and RAC2 and may also be involved in IL2 transcriptional activation via the activation of RAC2.
    • Cellular localizationIntracytoplasmic membrane. Peripheral membrane protein. Note=Colocalizes with F-actin.
    • Database links
    • Alternative names
      • Dedicator of cyto kinesis 2 antibody
      • Dedicator of cytokinesis 2 antibody
      • Dedicator of cytokinesis protein 2 antibody
      • DOCK 2 antibody
      • FLJ46592 antibody
      • KIAA0209 antibody
      see all

    Anti-DOCK2 antibody [EPR6647] images

    • All lanes : Anti-DOCK2 antibody [EPR6647] (ab124838) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 212 kDa
    • ab124838, at 1/50 - 1/100, staining DOCK2 in Human lung tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    References for Anti-DOCK2 antibody [EPR6647] (ab124838)

    ab124838 has not yet been referenced specifically in any publications.

    Product Wall

    You are correct, we observed better staining in IHC-P with ab124838 when no antigen retrieval was used. Thus, we recommend skipping the antigen retrieval step in your protocol. This may vary depending on your tissue type and processing methods, so it m...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"