Overview

  • Product nameAnti-DOCK4 antibody
    See all DOCK4 primary antibodies
  • Description
    Mouse monoclonal to DOCK4
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: NQVNEQSAPL PVPVPVPVPS YGGEEPVRKE SKTPPPYSVY ERTLRRPVPL PHSLSIPVTS EPPALPPKPL AARSSHLENG ARRTDPGPRP RPLPRKVSQL , corresponding to amino acids 1867-1967 of Human DOCK4

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 225 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in regulation of adherens junction between cells. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.
  • Tissue specificityWidely expressed at low level. Highly expressed in skeletal muscle, prostate and ovary.
  • Sequence similaritiesBelongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.
    Contains 1 SH3 domain.
  • DomainThe DHR-2 domain probably mediates the GEF activity.
  • Cellular localizationEndomembrane system.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dedicator of cytokinesis protein 4 antibody
    • Dock4 antibody
    • DOCK4_HUMAN antibody
    • KIAA0716 antibody

Anti-DOCK4 antibody images

  • DOCK4 antibody (ab56743) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer.


  • Predicted band size : 225 kDa
    DOCK4 antibody (ab56743) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab56743 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56743, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-DOCK4 antibody (ab56743)

ab56743 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"