Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling DOCK4 with ab85723 at 1/200 (1µg/ml). Detection: DAB.
Western blot - DOCK4 antibody (ab85723)
All lanes : Anti-DOCK4 antibody (ab85723) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 225 kDa Observed band size: 225 kDa
Exposure time: 30 seconds
Immunoprecipitation - DOCK4 antibody (ab85723)
Detection of Human DOCK4 by Immunoprecipitation in HeLa cell lysate (1 mg for IP, 20% of IP loaded)using ab85723 at 3ug/mg lysate. Subsequent Western blot detection was performed using 0.4 ug/ml ab85723. Detection:
Chemiluminescence with exposure times of 30 seconds.