Anti-DOCK7 antibody [3540C1a] (ab84866)
Key features and details
- Mouse monoclonal [3540C1a] to DOCK7
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-DOCK7 antibody [3540C1a]
See all DOCK7 primary antibodies -
Description
Mouse monoclonal [3540C1a] to DOCK7 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment with a tag, corresponding to internal sequence amino acids 1003-1112 of Human DOCK7
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Positive control
- Immunising recombinant Human DOCK7 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab84866 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
3540C1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 243 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 243 kDa. |
Target
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Relevance
DOCK7 functions as a guanine nucleotide exchange factor (GEF), which activates Rac1 and Rac3 Rho small GTPases by exchanging bound GDP for free GTP. It does not have a GEF activity for CDC42. It is required for STMN1 'Ser-15' phosphorylation during axon formation and consequently for neuronal polarization. -
Cellular localization
Cell projection, axon. Note=Enriched in the developing axons of hippocampal neurons. -
Alternative names
- 3110056M06Rik antibody
- dedicator of cytokinesis 7 antibody
- Dedicator of cytokinesis protein 7 antibody
see all
Images
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Anti-DOCK7 antibody [3540C1a] (ab84866) + Immunogen (Recombinant Human DOCK7 fragment)
Predicted band size: 243 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab84866 has not yet been referenced specifically in any publications.