Overview

  • Product name
  • Description
    Rabbit polyclonal to DOCK8
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 826-948 of Human DOCK8

  • Positive control
    • Human bone marrow tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab121177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.

Target

  • Function
    Potential guanine nucleotide exchange factor (GEF). GEF proteins activate some small GTPases by exchanging bound GDP for free GTP.
  • Involvement in disease
    Defects in DOCK8 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal recessive (AR-HIES) [MIM:243700]. It is a rare disorder of immunity characterized by immunodeficiency, recurrent infections, eczema, increased serum IgE, eosinophilia and lack of connective tissue and skeletal involvement.
  • Sequence similarities
    Belongs to the DOCK family.
    Contains 1 DHR-1 (CZH-1) domain.
    Contains 1 DHR-2 (CZH-2) domain.
  • Domain
    The DHR-2 domain may mediate some GEF activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200017A24Rik antibody
    • Dedicator of cytokinesis protein 8 antibody
    • DOCK8 antibody
    • DOCK8_HUMAN antibody
    • Epididymis luminal protein 205 antibody
    • HEL 205 antibody
    • MRD2 antibody
    • ZIR8 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus, nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121177 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121177 at 1/750 dilution staining DOCK8 in Paraffin-embedded Human bone marrow tissue by Immunohistochemistry.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

References

ab121177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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