• Product name
  • Description
    Rabbit polyclonal to DOCK9
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Opossum, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-50 of Human DOCK9 (NP_056111.1)

  • Positive control
    • HeLa whole cell lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/5000 - 1/15000. Detects a band of approximately 236 kDa (predicted molecular weight: 236 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.
  • Tissue specificity
    Widely expressed, with highest expression in heart and placenta. Expressed at intermediate level in kidney, brain, lung and skeletal muscle.
  • Sequence similarities
    Belongs to the DOCK family.
    Contains 1 DHR-1 domain.
    Contains 1 DHR-2 domain.
    Contains 1 PH domain.
  • Domain
    The DHR-2 domain is necessary and sufficient for the GEF activity.
  • Cellular localization
    Endomembrane system. Associated with membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdc42 guanine nucleotide exchange factor zizimin 1 antibody
    • Cdc42 guanine nucleotide exchange factor zizimin-1 antibody
    • Dedicator of cytokinesis protein 9 antibody
    • DKFZp686C11110 antibody
    • DKFZp686D2047 antibody
    • DKFZp686N04132 antibody
    • DOCK 9 antibody
    • DOCK9 antibody
    • DOCK9_HUMAN antibody
    • FLJ16744 antibody
    • FLJ44528 antibody
    • FLJ45282 antibody
    • FLJ45601 antibody
    • KIAA1058 antibody
    • KIAA1085 antibody
    • ZIZ1 antibody
    • Zizimin1 antibody
    see all

Anti-DOCK9 antibody images

  • All lanes : Anti-DOCK9 antibody (ab70272) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 236 kDa
    Observed band size : 236 kDa
    Additional bands at : 117 kDa,200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • HeLa whole cell lysate (1 mg) was immunoprecipitated with ab70272 at 3 µg/mg of lysate (lane 1) or with IgG control (lane 2). Samples were analysed by Western blotting using ab70272 at 0.04 µg/ml and detected by chemiluminescence with a 3 minute exposure time.
  • ICC/IF image of ab70272 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70272, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DOCK9 antibody (ab70272)

This product has been referenced in:
  • Wieczfinska J  et al. The whole-genome expression analysis of peripheral blood mononuclear cells from aspirin sensitive asthmatics versus aspirin tolerant patients and healthy donors after in vitro aspirin challenge. Respir Res 16:147 (2015). WB . Read more (PubMed: 26646719) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70272.
Please use the links above to contact us or submit feedback about this product.


Sign up