Overview

  • Product nameAnti-DOK1 antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    KTWAVLYPASPHGVAR

    conjugated to KLH, corresponding to amino acids 24-39 of Human DOK1.

  • Positive control
    • HL-60 cell lysate, Human breast carcinoma.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab37835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainThe PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 antibody images

  • Anti-DOK1 antibody (ab37835) at 1/100 dilution + HL-60 cell lysate.

    Secondary
    HRP-anti-rabbit

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab37835, at 1/1000 dilution, staining DOK1 in Human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-DOK1 antibody (ab37835)

ab37835 has not yet been referenced specifically in any publications.

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