Overview

  • Product nameAnti-DOK1 antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1
  • SpecificityDetects endogenous levels of total p62Dok protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human p62Dok around the phosphorylation site of tyrosine 362 (P-I-YP-D-E).

  • Positive control
    • JK cells, human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainThe PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 antibody images

  • All lanes : Anti-DOK1 antibody (ab47505)

    Lane 1 : extracts from JK cells,
    Lane 2 : extracts from JK cells, with immunizing peptide


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Ab47505 at 1/50 dilution staining paraffin-embedded human breast carcinoma tissue; left without and right with immunising peptide

References for Anti-DOK1 antibody (ab47505)

ab47505 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"