Overview

  • Product nameAnti-DOK1 antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 425-439 of Human DOK1 (Peptide available as ab39756.)

  • Positive control
    • Jurkat whole cell lysate
  • General notesTyrosine kinase substrate p62DOK


    Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with Human DOK1 peptide (ab39756).
ICC/IF Use a concentration of 2 µg/ml.
IP Use at an assay dependent dilution. (see Abreview published on 27th July, 2006).

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainThe PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 antibody images

  • All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml

    Lane 1 : Jurkat cell lysates
    Lane 2 : THP-1 cell lysate


    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF

References for Anti-DOK1 antibody (ab8112)

This product has been referenced in:
  • Siouda M  et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . Read more (PubMed: 24809689) »
  • Barrett A  et al. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci 127:2647-58 (2014). Read more (PubMed: 24762811) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Jurkat cells)
Total protein in input 2e+007 cells
Specification Jurkat cells
Immuno-precipitation step Protein A
Username

Mr. Guittard Geoffrey

Verified customer

Submitted Jul 27 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"