Recombinant
RabMAb

Anti-DOK1 antibody [EPR3228(2)] (ab155947)

Overview

  • Product name
    Anti-DOK1 antibody [EPR3228(2)]
    See all DOK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3228(2)] to DOK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human DOK1 (UniProt Q99704).

  • Positive control
    • HuT-78, K562 and Jurkat cell lysates; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
    • Tissue specificity
      Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
    • Sequence similarities
      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Domain
      The PTB domain mediates receptor interaction.
    • Post-translational
      modifications
      Constitutively tyrosine-phosphorylated.
      Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
    • Cellular localization
      Cytoplasm and Cytoplasm > perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • Docking protein 1 62kD antibody
      • Docking protein 1 antibody
      • DOK 1 antibody
      • DOK1 antibody
      • DOK1_HUMAN antibody
      • Downstream of tyrosine kinase 1 antibody
      • p62(dok) antibody
      • P62DOK antibody
      • pp62 antibody
      see all

    Images

    • All lanes : Anti-DOK1 antibody [EPR3228(2)] (ab155947) at 1/1000 dilution

      Lane 1 : HuT-78 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunofluorescent analysis of K562 cells labeling DOK1 with ab155947 at 1/250 dilution.

    References

    ab155947 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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