Anti-DOK1 (phospho S450) antibody (ab76172)

Overview

  • Product name
    Anti-DOK1 (phospho S450) antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1 (phospho S450)
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide corresponding to amino acids surrounding serine 450 from human DOK1, conjugated to KLH.

  • Positive control
    • Jurkat cells stimulated with calyculin
  • General notes
    Do not aliquot ab76172

Properties

Applications

Our Abpromise guarantee covers the use of ab76172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
ELISA 1/2000.

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificity
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similarities
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    The PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localization
    Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Images

  • All lanes : Anti-DOK1 (phospho S450) antibody (ab76172) at 1/1000 dilution

    Lane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min)
    Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min) followed by lambda phosphatase.


    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

References

ab76172 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76172.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up