Anti-DOK1 (phospho Y362) antibody (ab47374)

Overview

  • Product name
    Anti-DOK1 (phospho Y362) antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1 (phospho Y362)
  • Specificity
    Detects endogenous levels of p62Dok only when phosphorylated at tyrosine 362.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesised phosphopeptide derived from p62Dok around the phosphorylation site of tyrosine 362 (Human).

  • Positive control
    • Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab47374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificity
    Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similarities
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    The PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localization
    Cytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Images

  • All lanes : Anti-DOK1 (phospho Y362) antibody (ab47374) at 1/500 dilution

    Lane 1 : Extracts from JK cells. P-peptide (-)
    Lane 2 : Extracts from JK cells. P-peptide (+)


    Predicted band size : 52 kDa

References

ab47374 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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