Anti-DOK1 (phospho Y362) antibody (ab76173)

Overview

  • Product nameAnti-DOK1 (phospho Y362) antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1 (phospho Y362)
  • SpecificityThe sequence used as immunogen has high homology to DOK2 (Tyr-337). The site is not conserved in other DOK family members.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to amino acids surrounding tyrosine 362 in human DOK1, coupled to KLH.

  • Positive control
    • Jurkat cells stimulated with calyculin A (100 nM, 30 min).

Properties

Applications

Our Abpromise guarantee covers the use of ab76173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 52 kDa).
ELISA 1/2000.

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainThe PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y362) antibody images

  • All lanes : Anti-DOK1 (phospho Y362) antibody (ab76173) at 1/1000 dilution

    Lane 1 : Jurkat cells stimulated with calyculin A (100 nM, 30 min)
    Lane 2 : Jurkat cells stimulated with calyculin A (100 nM, 30 min), treated with alkaline phosphatase


    Predicted band size : 52 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-DOK1 (phospho Y362) antibody (ab76173)

ab76173 has not yet been referenced specifically in any publications.

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