Anti-DOK1 (phospho Y398) antibody (ab75741)

Overview

  • Product nameAnti-DOK1 (phospho Y398) antibody
    See all DOK1 primary antibodies
  • Description
    Rabbit polyclonal to DOK1 (phospho Y398)
  • Specificityab75741 detects endogenous levels of DOK1 only when phosphorylated at tyrosine 398.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human DOK1 around the phosphorylation site of tyrosine 398 (E-G-YP-E-L).

  • Positive control
    • Human breast carcinoma tissue. Extracts from K562 cells, starved.

Properties

Applications

Our Abpromise guarantee covers the use of ab75741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
  • Tissue specificityExpressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainThe PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway.
  • Cellular localizationCytoplasm and Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 1 62kD antibody
    • Docking protein 1 antibody
    • DOK 1 antibody
    • DOK1 antibody
    • DOK1_HUMAN antibody
    • Downstream of tyrosine kinase 1 antibody
    • p62(dok) antibody
    • P62DOK antibody
    • pp62 antibody
    see all

Anti-DOK1 (phospho Y398) antibody images

  • ab75741 at 1/50 dilution staining DOK1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-DOK1 (phospho Y398) antibody (ab75741) at 1/500 dilution

    Lane 1 : K562 cell extracts, unstarved
    Lane 2 : K562 cell extracts, starved


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-DOK1 (phospho Y398) antibody (ab75741)

ab75741 has not yet been referenced specifically in any publications.

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