Overview

  • Product name
  • Description
    Rabbit polyclonal to DOK2
  • Specificity
    Binds to a 55-60 kDa protein.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    20 amino acid C-terminal peptide to p56 dok2 RasGAP-binding protein

  • Positive control
    • Human U937 cells or T-cell line Jurkatt.

Properties

Applications

Our Abpromise guarantee covers the use of ab1677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000.
ELISA 1/100000.
ICC 1/300.

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • Sequence similarities
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Anti-DOK2 antibody images

  • Ab1677 staining human breast carcinoma. Staining is localized to nucleus and cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-DOK2 antibody (ab1677)

ab1677 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NMuMG)
Specification
NMuMG
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X100
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jun 16 2008

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NMuMG)
Loading amount
500000 cells
Specification
NMuMG
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 03 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up