The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1/50 - 1/100.
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Tissue specificityHighly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Sequence similaritiesBelongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain.
DomainPTB domain mediates receptor interaction.
Post-translational modificationsOn immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
ab37832, at 1/50 dilution, staining DOK2 in Human breast carcinoma tissue by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37832 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
References for Anti-DOK2 antibody (ab37832)
has not yet been referenced specifically in any publications.
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