Overview

  • Product name
  • Description
    Rabbit polyclonal to DOK2
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GWQPGT EYDNVVLKKG PK

    conjugated to KLH, corresponding to amino acids 395-412 of Human DOK2.

  • Positive control
    • CEM cell lysate, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab37832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • Sequence similarities
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Images

  • Anti-DOK2 antibody (ab37832) at 1/100 dilution + CEM cell lysate

    Secondary
    HRP-anti-rabbit

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab37832, at 1/50 dilution, staining DOK2 in Human breast carcinoma tissue by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab37832 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab37832 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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