Overview

  • Product name
  • Description
    Rabbit polyclonal to DOK2
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human DOK2 around the phosphorylation site of tyrosine 299 (G-E-YP-A-V).

  • Positive control
    • K562 cell extract, human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab47507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
  • Sequence similarities
    Belongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 2 56kDa antibody
    • Docking protein 2 antibody
    • DOK 2 antibody
    • DOK R antibody
    • DOK2 antibody
    • DOK2_HUMAN antibody
    • Downstream of tyrosine kinase 2 antibody
    • OTTHUMP00000161710 antibody
    • OTTHUMP00000224923 antibody
    • p56(dok 2) antibody
    • p56(dok-2) antibody
    • p56dok 2 antibody
    • p56DOK antibody
    • p56dok2 antibody
    see all

Images

  • Ab47507 at 1/50 dilution staining paraffin-embedded human breast carcinoma tissue; Left without and right with imunising peptide.

References

This product has been referenced in:
  • Ren Y  et al. Aberrant CD200/CD200R1 expression and its potential role in Th17 cell differentiation, chemotaxis and osteoclastogenesis in rheumatoid arthritis. Rheumatology (Oxford) N/A:N/A (2014). Read more (PubMed: 25261692) »
  • Li Y  et al. Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity. Arthritis Res Ther 14:R123 (2012). WB . Read more (PubMed: 22621248) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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