Overview

  • Product nameAnti-DOK3 antibody
    See all DOK3 primary antibodies
  • Description
    Goat polyclonal to DOK3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide:

    TRERRKGPAPCDRP

    , corresponding to C terminal amino acids 484-496 of Human DOK3 (NP_079148.1).

  • Positive control
    • Human PBMC lysate; Human placenta and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab118823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 53 kDa.
ELISA 1/64000.
IHC-P Use a concentration of 6.13 µg/ml.

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
  • Tissue specificityExpressed in spleen.
  • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainPTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Constitutively tyrosine-phosphorylated.
    On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
  • Cellular localizationCytoplasm. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Docking protein 3 antibody
    • DOK 3 antibody
    • DOK Like Protein antibody
    • Dok3 antibody
    • DOK3_HUMAN antibody
    • DOKL Pending antibody
    • Downstream of tyrosine kinase 3 antibody
    • p62 DOK Like Protein antibody
    see all

Anti-DOK3 antibody images

  • Anti-DOK3 antibody (ab118823) at 0.1 µg/ml + Human PBMC lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 53 kDa
  • ab118823, at 6.13 µg/ml, staining DOK3 in formalin fixed, paraffin embedded Human placenta tissue by Immunohistochemistry.
  • ab118823, at 6.13 µg/ml, staining DOK3 in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.

References for Anti-DOK3 antibody (ab118823)

ab118823 has not yet been referenced specifically in any publications.

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