Anti-DOK3 antibody (ab1357)
Key features and details
- Goat polyclonal to DOK3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOK3 antibody
See all DOK3 primary antibodies -
Description
Goat polyclonal to DOK3 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human DOK3 aa 450-550 (C terminal).
Database link: NP_079148.2 -
Positive control
- Human PBMC lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. -
Tissue specificity
Expressed in spleen. -
Sequence similarities
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
Domain
PTB domain mediates receptor interaction. -
Post-translational
modificationsConstitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase. -
Cellular localization
Cytoplasm. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 79930 Human
- Entrez Gene: 306760 Rat
- Omim: 611435 Human
- SwissProt: Q7L591 Human
- SwissProt: B2RYG7 Rat
- Unigene: 720849 Human
- Unigene: 19911 Rat
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Alternative names
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
see all
Images
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Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size: 57 kDaab1357 staining (1
Primary incubated for 1 hour. Detected by chemiluminescence.µ g/ml) of Human PBMC lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml
Lane 1 : Human Bone Marrow lysates
Lane 2 : Peripheral blood leukocyte lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
Datasheets and documents
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Datasheet download
References (3)
ab1357 has been referenced in 3 publications.
- Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
- Selimovic D et al. Apoptosis related protein-1 triggers melanoma cell death via interaction with the juxtamembrane region of p75 neurotrophin receptor. J Cell Mol Med 16:349-61 (2012). PubMed: 21418516
- Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586