The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
Tissue specificityExpressed in spleen.
Sequence similaritiesBelongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain.
DomainPTB domain mediates receptor interaction.
Post-translational modificationsConstitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size : 57 kDa
Primary incubated for 1 hour. Detected by chemiluminescence.
References for Anti-DOK3 antibody (ab1357)
This product has been referenced in:
Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics12:2874-89 (2013).
Read more (PubMed: 23820730) »
Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A106:1451-6 (2009).
Read more (PubMed: 19164586) »