Overview

  • Product nameAnti-DOK3 antibodySee all DOK3 primary antibodies ...
  • Description
    Mouse polyclonal to DOK3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human DOK3 protein (AAH04564.2; Q7L591-3).

  • Positive control
    • Human liver tissue lysate, DOK3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa for isoform 1 and 36 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
    • Tissue specificityExpressed in spleen.
    • Sequence similaritiesBelongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • DomainPTB domain mediates receptor interaction.
    • Post-translational
      modifications
      Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
    • Cellular localizationCytoplasm. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Docking protein 3 antibody
      • DOK 3 antibody
      • DOK Like Protein antibody
      • Dok3 antibody
      • DOK3_HUMAN antibody
      • DOKL Pending antibody
      • Downstream of tyrosine kinase 3 antibody
      • p62 DOK Like Protein antibody
      see all

    Anti-DOK3 antibody images

    • Anti-DOK3 antibody (ab89327) at 1 µg/ml + human liver tissue lysate at 50 µg

      Predicted band size : 36 kDa
      Observed band size : ~58 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DOK3 antibody (ab89327) at 1 µg/ml

      Lane 1 : DOK3 transfected 293T cell lysate
      Lane 2 : Non transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 36 kDa

    References for Anti-DOK3 antibody (ab89327)

    ab89327 has not yet been referenced specifically in any publications.

    Product Wall

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