The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa for isoform 1 and 36 kDa for isoform 2.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
Tissue specificityExpressed in spleen.
Sequence similaritiesBelongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain.
DomainPTB domain mediates receptor interaction.
Post-translational modificationsConstitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.