General notesab94169 is a 293T cell transfected lysate in which Human DOK3 has been transiently over-expressed using a pCMV-DOK3 plasmid. The lysate is provided in 1X Sample Buffer.
Note: For more detailed about how the transfected lysate was prepared view preparation notes
BackgroundFunction: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
Tissue specificity: Expressed in spleen.
Similarity: Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
Domain: PTB domain mediates receptor interaction.
PTM: Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.