Overview

  • Product name
  • Description
    Goat polyclonal to DOK5
  • Specificity
    This antibody is expected to recognise both reported isoforms of DOK5.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    LHRTETFPA YRSEH

    , corresponding to C terminal amino acids 293-306 of Human DOK5

  • Positive control
    • Human kidney, muscle and thymus lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab24360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:16,000.
    IHC-P: Use at a concentration of 1 - 2 µg/ml. In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells.
    WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 26-28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
    • Tissue specificity
      Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
    • Sequence similarities
      Belongs to the DOK family. Type B subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Domain
      PTB domain mediates receptor interaction.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.
    • Information by UniProt
    • Database links
    • Alternative names
      • chromosome 20 open reading frame 180 antibody
      • Docking protein 5 antibody
      • DOK 5 antibody
      • DOK5 antibody
      • DOK5 protein antibody
      • DOK5_HUMAN antibody
      • Downstream of tyrosine kinase 5 antibody
      • Insulin receptor substrate 6 antibody
      • IRS-6 antibody
      • IRS6 antibody
      see all

    Images

    • Anti-DOK5 antibody (ab24360) at 0.1 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Observed band size : 27 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 hour
    • Ab24360 (1ug/ml) staining human DOK5 in human tonsil by immunohistochemistry using paraffin embedded tissue.

      Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

    References

    This product has been referenced in:
    • Pan Y  et al. Dok5 is involved in the signaling pathway of neurotrophin-3 against TrkC-induced apoptosis. Neurosci Lett 553:46-51 (2013). Read more (PubMed: 23954828) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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