The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1:16,000.
IHC-P: Use at a concentration of 1 - 2 µg/ml. In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells.
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 26-28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
Belongs to the DOK family. Type B subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain.
PTB domain mediates receptor interaction.
Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.