Overview

  • Product nameAnti-DOK5 antibody
    See all DOK5 primary antibodies
  • Description
    Rabbit polyclonal to DOK5
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: SKGPKRLEKF SDERAAYFRC YHKVTELNNV KNVARLPKST KKHAIGIYFN, corresponding to amino acids 36-85 of Human DOK5 (NP_060901).

  • Positive control
    • Placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • FunctionDOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
  • Tissue specificityHighest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
  • Sequence similaritiesBelongs to the DOK family. Type B subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • DomainPTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.
  • Information by UniProt
  • Database links
  • Alternative names
    • chromosome 20 open reading frame 180 antibody
    • Docking protein 5 antibody
    • DOK 5 antibody
    • DOK5 antibody
    • DOK5 protein antibody
    • DOK5_HUMAN antibody
    • Downstream of tyrosine kinase 5 antibody
    • Insulin receptor substrate 6 antibody
    • IRS-6 antibody
    • IRS6 antibody
    see all

Anti-DOK5 antibody images

  • Anti-DOK5 antibody (ab80367) at 1 µg/ml ( in 5% skim milk / PBS buffer) + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa

References for Anti-DOK5 antibody (ab80367)

ab80367 has not yet been referenced specifically in any publications.

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