Anti-DOK5 antibody - Aminoterminal end (ab71308)

Overview

  • Product name
    Anti-DOK5 antibody - Aminoterminal end
    See all DOK5 primary antibodies
  • Description
    Rabbit polyclonal to DOK5 - Aminoterminal end
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Positive control
    • Mouse liver tissue lysate, human cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/1000.

Target

  • Function
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK5 functions in RET-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of RET in neuronal differentiation.
  • Tissue specificity
    Highest expression in skeletal muscle, lower in brain, heart and kidney. Also detected in activated peripheral blood T-lymphocytes.
  • Sequence similarities
    Belongs to the DOK family. Type B subfamily.
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Domain
    PTB domain mediates receptor interaction.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to insulin, IGF1 and GDNF.
  • Information by UniProt
  • Database links
  • Alternative names
    • chromosome 20 open reading frame 180 antibody
    • Docking protein 5 antibody
    • DOK 5 antibody
    • DOK5 antibody
    • DOK5 protein antibody
    • DOK5_HUMAN antibody
    • Downstream of tyrosine kinase 5 antibody
    • Insulin receptor substrate 6 antibody
    • IRS-6 antibody
    • IRS6 antibody
    see all

Images

  • Anti-DOK5 antibody - Aminoterminal end (ab71308) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

    Predicted band size : 35 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,43 kDa,80 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacting with ab71308, peroxidase-conjugated secondary used, followed by AEC staining.

References

ab71308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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