Donkey Anti-Chicken IgY H&L (FITC) (ab63507)

Overview

  • Product nameDonkey Anti-Chicken IgY H&L (FITC)
    See all IgY secondary antibodies
  • Target speciesChicken
  • Tested applicationsSuitable for: IHC-Frmore details
  • Immunogen

    Purified chicken IgY isolated from normal chicken egg yolks.

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein L purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.

Donkey Anti-Chicken IgY H&L (FITC) images

  • ab13970 staining GFP in E10.5 mouse embryo sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBS/0.5% Triton X100 and blocked with protein block for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 3 hours at 25°C. A FITC-conjugated donkey anti-chicken IgY H&L (ab63507) (1/500) was used as the secondary antibody.

    See Abreview

  • Ab63507 was used at dilution 1/100 with the primary antibody ab13970 in IHC - Free Floating. See the review on ab13970.

References for Donkey Anti-Chicken IgY H&L (FITC) (ab63507)

This product has been referenced in:
  • Ribera A  et al. Biochemical, histological and functional correction of mucopolysaccharidosis type IIIB by intra-cerebrospinal fluid gene therapy. Hum Mol Genet 24:2078-95 (2015). IHC-P . Read more (PubMed: 25524704) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
Antigen retrieval is optional in this staining.
Primary antibody: Anti-GFP antibody (ab13970) 1:800 at 4 degree overnight.
Secondary antibody: Donkey Anti-Chicken (FITC) ab63507 at RT 4h (long incubation time of secondary would help to increase the signal).
Username

Abcam user community

Verified customer

Submitted Feb 25 2014

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numero...

Read More
Application Immunohistochemistry free floating

Sample: Mouse Tissue sections (Brain and spinal cord)
Specification: Brain and spinal cord

Other product details
Dilution: 1/500
Incubation time: 12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody
Secondary antibody: None used

Additional data
Additional Notes: Please note that the below average score represents a score for an application that was not under the tested applications list on the information sheet.
Abcam response: Thank you for submitting feedback on this antibody in an untested application.
Username

Abcam user community

Verified customer

Submitted Nov 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"