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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
Overview
Product name
Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed
See all IgG products (1691) ...
Target species
Rabbit
Tested applications
IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Immunogen
Rabbit IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C in the dark. Do not freeze.
Storage buffer
Preservative: None, 0.05% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, pH 7.6
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins
Conjugation notes
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Fluorophore >> Phycoerythrin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-P: 1/200IHC-P: 1/200
ICC/IF: 1/100 - 1/250.
IM: Use at an assay dependent dilution.
Flow Cyt: 1/100 - 1/250.(The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. )
Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed images:
Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
Ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
Ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.
References for Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
This product has been referenced in:
- Kästle Met al. Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes. Free Radic Biol Med 50:305-12 (2011). Flow Cyt.Read more (PubMed: 21078385) »
- Jones CIet al. A functional genomics approach reveals novel quantitative trait loci associated with platelet signaling pathways. Blood 114:1405-16 (2009). Flow Cyt.Read more (PubMed: 19429868) »
See all 5 publications for this product
Publishing research using ab7007? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"