Overview

  • Product nameDonkey F(ab) Anti-Goat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesGoat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
  • Tested applicationsSuitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Donkey IgG Fab molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Dot blot
WB
ELISA
IHC
Immunomicroscopy
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/8000 - 1/36000.
    IHC: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.

    This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/8000 to 1/36000 of the reconstitution concentration is suggested for this product.

    Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey F(ab) Anti-Goat IgG H&L (Biotin) (ab6578)

    This product has been referenced in:
    • Graham BB  et al. Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am J Pathol 177:1549-61 (2010). IHC-P . Read more (PubMed: 20671265) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab6578.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"