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Product name Donkey F(ab) Anti-Goat IgG H&L (FITC) See all IgG secondary antibodies
Target species Goat
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c).
Tested applications Suitable for:
ICC/IF, Flow Cyt, ELISA more details
Goat IgG whole molecule
Conjugation FITC. Ex: 493nm, Em: 528nm Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration information loading...
Purification notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notes Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons)
Absorption Wavelength: 495 nm Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Donkey IgG Fab
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes Flow Cyt: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Donkey F(ab) Anti-Goat IgG H&L (FITC) (ab6521)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"