Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons)
Absorption Wavelength: 596 nm
Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio: 1.0 moles Texas Red per mole of Donkey IgG Fab
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Donkey F(ab) Anti-Goat IgG H&L (Texas Red ®) (ab6523)
This product has been referenced in:
Carozzi VA et al. Expression, distribution and glutamate uptake activity of high affinity-excitatory aminoacid transporters in in vitro cultures of embryonic rat dorsal root ganglia. Neuroscience : (2011).
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