Overview

  • Product nameDonkey F(ab) Anti-Goat IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Target speciesGoat
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c).
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • Immunogen

    Goat IgG whole molecule

  • ConjugationTexas Red ®. Ex: 596nm, Em: 620nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.0 moles Texas Red per mole of Donkey IgG Fab
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey F(ab) Anti-Goat IgG H&L (Texas Red ®) (ab6523)

    This product has been referenced in:
    • Carozzi VA  et al. Expression, distribution and glutamate uptake activity of high affinity-excitatory aminoacid transporters in in vitro cultures of embryonic rat dorsal root ganglia. Neuroscience : (2011). ICC/IF . Read more (PubMed: 21726604) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab6523.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"