Overview

  • Product name
    Donkey F(ab) Anti-Goat IgG H&L (Texas Red ®)
    See all IgG secondary antibodies
  • Target species
    Goat
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c).
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, Immunomicroscopy, ELISAmore details
  • Immunogen

    Goat IgG whole molecule

  • Conjugation
    Texas Red ®. Ex: 596nm, Em: 620nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
  • Conjugation notes
    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.0 moles Texas Red per mole of Donkey IgG Fab
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Flow Cyt
Immunomicroscopy
ELISA
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    IM: Use at an assay dependent dilution.

    Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Donkey F(ab) Anti-Goat IgG H&L (Texas Red ®) (ab6523)

    This product has been referenced in:
    • Carozzi VA  et al. Expression, distribution and glutamate uptake activity of high affinity-excitatory aminoacid transporters in in vitro cultures of embryonic rat dorsal root ganglia. Neuroscience : (2011). ICC/IF . Read more (PubMed: 21726604) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab6523.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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