Donkey F(ab) Anti-Rabbit IgG H&L (Biotin) (ab102281)

Overview

  • Product nameDonkey F(ab) Anti-Rabbit IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: WB, ELISA, ICC, IHC-Pmore details
  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab) fragments were generated using a papain digestion. F(ab')2, Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/200000. (Chemiluminescence)
ELISA 1/10000 - 1/200000. (Primary)
ICC 1/200 - 1/1000.
IHC-P 1/500 - 1/5000.

References for Donkey F(ab) Anti-Rabbit IgG H&L (Biotin) (ab102281)

ab102281 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"