Donkey F(ab) Anti-Rabbit IgG H&L (HRP) (ab102283)

Overview

  • Product nameDonkey F(ab) Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: WB, ELISA, ICC, IHC-Pmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab) fragments were generated using a papain digestion. F(ab')2, Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to HRP.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/30000. (Colorimetric) or 1/10000 - 1/50000 (Chemiluminescence)
ELISA 1/10000 - 1/100000. (Primary)
ICC 1/200 - 1/5000.
IHC-P 1/200 - 1/5000.

References for Donkey F(ab) Anti-Rabbit IgG H&L (HRP) (ab102283)

ab102283 has not yet been referenced specifically in any publications.

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We recommend not to store this antibody at -20C. In our experience the conjugated antibodies should not be frozen because the conjugate lost reactivity quite often.

I would suggest thawing the antibody onc...

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Thank you very much for your question.

According to the lab, all of the cysteines in the F(ab) fragment should be in five pairs of disulfide bonds. There should be no free cysteines or thiols unless you do a partial reduction to break some of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"