Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)

Overview

  • Product nameDonkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, mouse, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Mouse, Rabbit, Rat, Sheep more details
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, horse, mouse, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.

References for Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab102439)

ab102439 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5min) and binding of remicade was detected with PE Donkey F(ab)2 anti-hIgG H&L (ab102439) – 1:100 (5ug/ml), 30 min incubation in 4°C.

PE donkey F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal and displaying minimal non-specific binding the cells alone, non-treated with remicade.
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Submitted Apr 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"