Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

Overview

  • Product nameDonkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesHuman
  • Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Human IgG whole molecule

  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ClonalityPolyclonal
  • General notes


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

References for Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

ab7005 has not yet been referenced specifically in any publications.

Product Wall

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means...

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I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means...

Read More

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"