Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed (ab98769)

Overview

  • Product nameDonkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsFlow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • ConjugationDyLight® 650. Ex: 654nm, Em: 673nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed images

  • ab18005 staining phosphatidylserine in rat carotid artery tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with 50% ethanol in TPBS and blocked with 15% normal horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in 15% normal horse serum) for 18 hours at 4°C. A DyLight® 650-conjugated Donkey F(ab')2 anti-mouse IgG H&L (ab98769) (1/500) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 650), pre-adsorbed (ab98769)

ab98769 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"