Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

Overview

  • Product nameDonkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibodies:
    Mouse anti-disabled-2 (endoderm marker), 1:100
    Rabbit anti-lamin a 1 (basement membrane marker), 1:400

    Secondary antibodies:
    Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG -H&L (DyLight® 488), pre-adsorbed (ab98766), 1:200
    Goat anti-rabbit IgG Cy3®, 1:200

    Nuclei were counterstained with DAPI
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

ab98766 has not yet been referenced specifically in any publications.

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I can confirm it is not unusual for an antibody to be kept at 4oC. The datasheet for many of our antibodies recommend to store at this temperature. At this temperature, they should be stable for at least 1 year. (...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"