Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 594), pre-adsorbed (ab98768)

Overview

  • Product name
    Donkey F(ab')2 Anti-Mouse IgG - H&L (DyLight® 594), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep more details
  • Conjugation
    DyLight® 594. Ex: 593nm, Em: 618nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 594.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab98768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Flow Cytometry
TE11 cells or human PBMC cells were stained with anti-HLA Class-I antibody (ab23755) and anti-mouse IgG DyLight594 (ab98768), followed by FACS analysis.
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Thank you for contacting us.

I think this information would need to be obtained from the manufacturer of the microscope and filters that the customer is planning touse. They would be able to exactly tell the customer which wavelengths can be...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up