Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (ab150070)

Overview

  • Product name
    Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Minimal
    cross-reactivity

    Human, Mouse, Rat more details
  • Conjugation
    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 68% PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration. Use at an assay dependent dilution
IHC-P Use at an assay dependent concentration. Use at an assay dependent dilution
Flow Cyt 1/2000.

Images

  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150070, Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The secondary antibody only control displays negative staining, as expected.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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