For the best experience on the Abcam website please upgrade to a modern browser such as
Product name Donkey F(ab')2 Anti-Rabbit IgG - H&L (AP), pre-adsorbed See all IgG secondary antibodies
Target species Rabbit
Tested applications Suitable for:
ICC, ELISA, IHC-P, WB more details
Minimal cross-reactivity Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Conjugation Alkaline Phosphatase Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Alkaline Phosphatase.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/1000 - 1/10000. (Primary)
1/100 - 1/1000.
1/1000 - 1/10000. (Chemiluminescent)
References for Donkey F(ab')2 Anti-Rabbit IgG - H&L (AP), pre-adsorbed (ab98487)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"