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Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
Purity Immunogen affinity purified
Purification notes This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Fluorescein.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/500.
1/50 - 1/500.
1/50 - 1/200.
Donkey F(ab')2 Anti-Rabbit IgG H&L (FITC) images
Immunohistochemistry (Frozen sections) - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab98439) Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
Fix in 3%PFA in PBS for 30 min at RT
Incubate in 7.5% sucrose-PBS for 3h at RT
Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
Embed the EBs in tissue-Tek OCT compound
Cut frozen sections to 4-20 µm thickness
Primary antibody: Rabbit anti-laminin alpha 1 (basement membrane marker), 1:400
Secondary antibody: Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98439), 1:100
Nuclei were counterstained with DAPI
F-actin was stained with rhodamine-phalloidin, 1:100
References for Donkey F(ab')2 Anti-Rabbit IgG H&L (FITC) (ab98439)
has not yet been referenced specifically in any publications.
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