Donkey F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed (ab98493)

Overview

  • Product name
    Donkey F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details
  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/5000 - 1/30000. (colorimetric), 1/10000-1/50000 (chemiluminescent).

References

This product has been referenced in:
  • Satomoto M  et al. Sugammadex-Enhanced Neuronal Apoptosis following Neonatal Sevoflurane Exposure in Mice. Anesthesiol Res Pract 2016:9682703 (2016). WB, IHC . Read more (PubMed: 27895665) »
  • Cui W  et al. Glial dysfunction in the mouse habenula causes depressive-like behaviors and sleep disturbance. J Neurosci 34:16273-85 (2014). Read more (PubMed: 25471567) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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