Donkey F(ab')2 Anti-Rabbit IgG - H&L, pre-adsorbed (ab98486)

Overview

  • Product name
    Donkey F(ab')2 Anti-Rabbit IgG - H&L, pre-adsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC, ELISA, IHC-P, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/100 - 1/30000. Coating: Use 1/100 - 1/500 dilution; Primary: Use 1/1000 - 1/30000 dilution.
IHC-P 1/200 - 1/2000.
WB 1/1000 - 1/30000. Colorimetric: Use 1/1000 - 1/10000 dilution; Chemiluminescent: Use 1/1000 - 1/30000 dilution.

References

ab98486 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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