Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232)

Overview

  • Product nameDonkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
  • Tested applicationsSuitable for: IHC-P, ICC, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase absorbed to ensure class specificity. Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-perlecan (basement membrane marker), 1:100
    Secondary antibody: Donkey F(ab')2 polyclonal to Rat IgG - H&L DyLight® 488 (ab102232), 1:200
    Nuclei were counterstained with DAPI

References for Donkey F(ab')2 Anti-Rat IgG - H&L (DyLight® 488), pre-adsorbed (ab102232)

This product has been referenced in:
  • Xia F  et al. IRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis. Sci Rep 3:1867 (2013). Read more (PubMed: 23689639) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"