Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)

Overview

  • Product nameDonkey Anti-Goat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP)
  • Target speciesGoat
  • Tested applicationsDot Blot, ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    Full length native Goat IgG (purified).

  • ConjugationAlkaline Phosphatase

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 50mM Tris HCl, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads.
  • Conjugation notesAlkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
ELISA 1/8300.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.
WB 1/500 - 1/2500.

Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) images

  • Ab6886 was used at dilution 1/2500 with the primary antibody ab7613 in WB. See the review on ab7613.

References for Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab6886)

ab6886 has not yet been referenced specifically in any publications.

Product Wall

The secondary antibody ab6886 will recognize the anti-Myc tag antibody (ab9132) which you are using. This secondary is Alkaline Phosphatase conjugated, in order to detect this in Western Blot you will need to use a substrate which will react with A...

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Thank you for contacting us.

As requested, I have attached the Certificates of Compliance for the antibodies and reagents you mentioned - to this email.

I hope this information is helpful to you. Please do not hesitate to contact ...

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Vielen Dank für Ihre Anfrage.

Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

Drei der...

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Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"