Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107)

Overview

  • Product name
    Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target species
    Goat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notes
    Molar enzyme: antibody protein ratio is 1:1
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Part of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. (Primary).
WB 1/5000 - 1/50000. (Chemiluminescent).

Images

  • All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time : 8 minutes

References

ab97107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us with your question. For the alkaline phosphatase conjugated antibody ab97107, you can use the liquid fast-red kit ab64254- http://www.abcam.com/Liquid-Fast-Red-Substrate-Kit-ab64254.html For the HRP conjugated antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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