Donkey Anti-Goat IgG H&L (Biotin) (ab6884)
Key features and details
- Donkey Anti-Goat IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Donkey
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
Overview
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Product name
Donkey Anti-Goat IgG H&L (Biotin)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Goat -
Tested applications
Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
Immunogen
Full length native Goat IgG (purified).
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Use donkey anti-goat IgG (H&L) biotin to confidently detect primary antibodies raised in goat in your immunohistochemistry, western blot, ELISA or immunoprecipitation. Get dilutions to get the best results in your IHC, WB, IP, or ELISA with donkey anti-goat IgG (H&L) biotin below.Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000 - 1/5000.
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Dot blot |
Use at an assay dependent dilution.
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ELISA |
1/180000.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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Immunomicroscopy |
Use at an assay dependent dilution.
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WB |
1/2000 - 1/10000.
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Notes |
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ICC/IF
1/1000 - 1/5000. |
Dot blot
Use at an assay dependent dilution. |
ELISA
1/180000. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
Immunomicroscopy
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
Images
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ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Micr
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab6884 has been referenced in 23 publications.
- Gaff J et al. Expression in skin biopsies supports genetic evidence linking CAMKK2, P2X7R and P2X4R with HIV-associated sensory neuropathy. J Neurovirol 29:241-251 (2023). PubMed: 37166584
- Ninčević V et al. Renoprotective Effect of Liraglutide Is Mediated via the Inhibition of TGF-Beta 1 in an LLC-PK1 Cell Model of Diabetic Nephropathy. Curr Issues Mol Biol 44:1087-1114 (2022). PubMed: 35723295
- Han Q et al. Targeted inhibition of SIRT6 via engineered exosomes impairs tumorigenesis and metastasis in prostate cancer. Theranostics 11:6526-6541 (2021). PubMed: 33995674
- Nguyen L et al. Survival and Motor Phenotypes in FVB C9-500 ALS/FTD BAC Transgenic Mice Reproduced by Multiple Labs. Neuron 108:784-796.e3 (2020). PubMed: 33022226
- Jeong D et al. LRIG1-Mediated Inhibition of EGF Receptor Signaling Regulates Neural Precursor Cell Proliferation in the Neocortex. Cell Rep 33:108257 (2020). PubMed: 33053360